Psicheck-2 vector

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August undefined, 2024

WebBy cloning the 3’UTRs of collagen IV subunits into the psiCHECK TM-2 vector, we found that seven of the eight sites in the Col4a3 – Col4a6 gene complementary to miR-29 were … WebpsiCHECK-2 Description psiCHEC-2 Vector is designed to provide a quantitative and rapid approach for optimization of RNA interference (RNAi). The vectors enable the monitoring …

Dual Luciferase Reporter Vector Psicheck 2 Promega Bioz

WebThere are two siCHECK™ Vectors, the psiCHECK™-1 Vector and the psiCHECK™-2 Vector. Both vectors contain as the primary reporter gene the synthetic version of Renilla … WebVector Sequences; Software & Firmware . Downloads; Support. Global Support . Our customer and technical support experts are here to help! Explore Support Center; Medical Affairs . We provide medical information and facilitate research collaborations. ... psiCHECK™-2 Vector field church mandeville

Psicheck 2 Vector Promega Bioz

WebMCF-7 cells were transfected with a reporter vector psiCHECK2-FSCN1 3'UTR or psiCHECK2 control vector and miR-133a mimic or negative control. Each transfection was carried out in triplicate. Luciferase assay was performed 48 h after gene transfection. Firefly luciferase activity was normalized to Renilla luciferase activity. WebFeb 16, 2024 · PsiCHECK-2 vector contained the ampicillin (Amp) resistance gene. The recombinant plasmids were obtained through transformation of Escherichia coli (E. coli) DH5a bacteria as a host. greyhound west palm beach station

Light Up Your Best shRNA - Promega

WebJul 7, 2024 · In this study, we replaced the SV40 promoter in the psiCHECK-2 reporter vector, which is widely used with mammalian cell lines, with the HSV-TK or AC5.1 … WebDec 18, 2024 · Wild-type or mutant circCHST15 or 3′-UTR EIF4EBP1 was synthesized and then subcloned into psiCHECK-2 vector. HEK293T cells were seeded in 24-well plates at a concentration of 1.5 × 105 per well and cotransfected miR-125a-5p mimics or miR-NC with psiCHECK-2-wt-circCHST15, psiCHECK-2-mut-circCHST15, psiCHECK-2-wt-EIF4EBP1 or … greyhound welpenWebDual Luciferase Reporter Gene Vector Psicheck 2, supplied by Promega, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, … field chronograph watches

"WebThe psiCHECK-2 Vector is a dual Luciferase vector designed to provide a quantitative and rapid approach for initial optimization of RNA interference (RNAi). The vector enable … " - Psicheck-2 vector

Psicheck-2 vector

Significant modulations of linc001128 and linc0938 with miR-24

WebThe psiCHECK™-1 Vector and psiCHECK™-2 Vector provide a quantitative and rapid approach to optimize RNA interference (RNAi). The vectors enable monitoring of changes in expression of a target gene fused to the reporter gene. WebVector Sequences; Software & Firmware . Downloads; Support. Global Support . Our customer and technical support experts are here to help! Explore Support Center; Medical …

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WebpsiCHECK-1 and psiCHECK-2 Vectors enable monitoring of changes in expression of a target gene fused to a reporter gene. They are used for optimizing RNA interference … WebThe psiCHECK™-2 Vector is designed to provide quantitative and rapid optimization of RNA interference (RNAi). The vector enables monitoring of changes in expression of a target …

WebpsiCHECK-2 Description psiCHEC-2 Vector is designed to provide a quantitative and rapid approach for optimization of RNA interference (RNAi). The vectors enable the monitoring of changes in expression of a target gene fused to the reporter gene. WebMay 5, 2009 · UTR sequences, up to and including the primary ATG initiation codon, were synthesized (IDT), cloned and ligated into the NheI site directly preceding the Renilla luciferase gene in the dual-luciferase vector psiCHECK-2 (Promega) .

WebpsiCHECK-1 and psiCHECK-2 Vectors enable monitoring of changes in expression of a target gene fused to a reporter gene. They are used for optimizing RNA interference … WebpsiCHECK2 (Search Vector Database) Backbone manufacturer Promega Backbone size (bp) 6273 Modifications to backbone Insertion of a SalI/NotI/XhoI linker between the NotI and …

WebpsiCHECK2 (Search Vector Database) Backbone manufacturer Promega Backbone size (bp) 6273 Modifications to backbone Insertion of a SalI/NotI/XhoI linker between the NotI and XhoI site Vector type Mammalian Expression Growth in Bacteria Bacterial Resistance (s) Ampicillin, 100 μg/mL Growth Temperature 37°C Growth Strain (s) DH5alpha Copy number

WebThe psiCHECK™-2 Vector is designed to provide quantitative and rapid optimization of RNA interference (RNAi). The vector enables monitoring of changes in expression of a target … field church ipswichWebThe regulation of DPC4 by miR-190 was evaluated by qRT-PCR, Western blotting, and luciferase reporter assays in the human CRC cell line HT-29 after treatment with miR-190 mimics and inhibitors. Results: The DPC4 mRNA, miR-, and DPC4 protein expression levels were highest in LS174T cells while lowest in SW480 and SW620 cells. field chronographWebpsiCHECK™-2: miR-21 Biosensor No light Figure 1. Detection of miR-21 activity using the psiCHECK™-2 Vector. miR-21 target sequence was cloned into the multiple cloning … field chrysler