Phleomycin plates
WebbPhleomycin-resistant transformants are selected with 10-50µg/mL of phleomycin in the regeneration medium, depending on the sensitivity of the host strain. Selectivity can be … WebbSerial dilutions of each strain were plated onto YEPD, MMS plates (0.003% and 0.004%), and phleomycin plates (3 g/ml and 6 g/ml). To measure UV sensitivity, ...
Phleomycin plates
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WebbZeocin即phleomycin D1，中文名称博来霉素或者腐草霉素D1，是从轮状链霉菌(Streptomyces verticillus)的一个突变体菌株中分离而来的博 ... WebbPhleomycin is a glycopeptide antibiotic and is a member of the bleomycin family. Application Phleomycin from Streptomyces verticillus has been used as an antibiotic in …
Webb26 apr. 2024 · Finally, the colonies were tested for sensitivity towards phleomycin by transferring them using sterile toothpicks to YE-phleomycin plates. If colonies were unable to grow on these plates, it was an indication that the yeast cells eliminated the pUT332 plasmid during the non-selective prolonged growth period. Webb2 dec. 2010 · PDF Sectoring patterns of mutants in the rad52 epistasis group. Top panels: 5ORIΔ-ΔR chromoductants of rad50Δ, rad51Δ, rad52Δ, rad54Δ, rad55Δ, rad57Δ,... Find, read and cite all the ...
WebbThe phleomycin-resistant cells ( Fig. 9.6) were obtained in an average of 1/1.6 plates through selection for 3–4 weeks, with a transformation efficiency of approximately 5.5 × … Webb5-alpha Competent E. coli cells were transformed with 2 µl of the reaction sample following the Gibson Assembly Transformation Protocol from NEB. Next, 100 µl of the cells were plated onto LB medium plates with Phleomycin at a concentration of 20 ug/ml. The plates were incubated overnight at 37 °C. Figure 9.
WebbDescription Zeocin™ Selection Reagent is a formulation of phleomycin D1, a basic, water-soluble, copper-chelated glycopeptide isolated from Streptomyces verticillus. The …
Webb(C) Representative fluorescence micrographs showing colocalization of Mte1 with Mph1 following phleomycin treatment. The mCherry, GFP, and merged images are shown. (D) … inchbug after shark tankWebb29 mars 2012 · Verification of mutant strains by drug sensitivity. (a) Phleomycin plates were incubated for 3 days at 30°C for S. cerevisiae and 5 days at 23°C for S. bayanus. inappropriate awardsWebb29 apr. 2024 · Given phleomycin causes global DSBs by an unknown mechanism, we also tested the effects of deletion of DMA1 and DMA2 on plates containing an established … inchbrook nailsworthWebb29 dec. 2008 · After mixing, regenerated protoplasts were spread on square plates containing 40 ml of solidified MM. Regeneration medium contained no antibiotic, 25 μg of phleomycin ml −1, or 10 μg of nourseothricin ml −1. Selection plates were incubated at 30°C for 5 days. Isolation of genomic DNA and RNA. inappropriate attire for womenWebbhygromycin B and phleomycin were purchased from Cayla. For efficient selection, some batches of hygromycin B required higher concentrations (up to 400 µg/ml). Phleomycin … inchbug codeWebbfrom the original plate selected on 50 µg/ml of phleomy-cin maintained resistance to phleomycin. Conversely, 11 of 18 colonies selected on 100 µg/ml of phleomycin … inchbug couponWebb30 nov. 2015 · Plates were photographed after 2 to 3 days. (E) The indicated strains were cultured in the presence of the indicated concentrations of phleomycin for 2 hours, diluted and plated inappropriate attire for workplace