Flp-in cho
WebA single gene copy of shRNA-2 also decreased the translational level of DHFR by 80% in Flp-In CHO cells. shRNA-2 was then incorporated into a plasmid vector expressing human erythropoietin (EPO) and an exogenous DHFR to develop … WebJan 13, 2024 · HEK & CHO mammalian cell lines in comparison. CHO mammalian cell lines have, similar to HEK cells, a long history of being used for the production of various …
Flp-in cho
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WebFRT site in the Flp-In™-CHO™ cell line has not been mapped, but has been demonstrated to have integrated into a highly transcriptionally active genomic locus as determined by … WebJul 17, 2024 · In order to test the Flp-in tm-ready cells, Lipofectamine 2000 was used to transfect 5 × 10 4 Lec 3.2.8.1 or 4 × 10 5 DG44 CHO cells with our GOI, in this case 100 ng of circular AQP3-GFP_pcDNA5/FRT …
WebDiagnostic Tests. ANKLE/BRACHIAL INDEX. BLOOD PRESSURE TEST. CARDIAC CATHETERIZATION. CARDIAC STRESS TESTING. CAROTID ULTRASOUND. CORONARY CALCIUM SCAN. CORONARY COMPUTED TOMOGRAPHY ANGIOGRAPHY (CCTA) ECHOCARDIOGRAPHY.
WebLike Cre, FLP is a tyrosine recombinase, but it originates from the yeast Saccharomyces cerevisiae, rather than from bacteriophage. In FLP-FRT, FLP is analogous to cre and FRT to loxP – FLP recognizes the FRT sites, and catalyzes recombination between them. WebNov 11, 2013 · Flip-In CHO cells (FCHO) stably transfected by plasmid DNA were stained by PE-conjugated mouse-anti-human kappa chain antibodies (PE-Kappa) and then analyzed by FACS. ... Invitrogen (2006) Flp-In system for generating stable mammalian expression cell lines by Flp recombinase-mediated integration. Instruction Manual. Carlsbad, CA. 22.
WebJun 21, 2024 · Among different recombinases, the Flp/FRT system has been extensively applied in CHO cells to express monoclonal antibodies 35 and recombinant proteins 34, 36 probably due to its high...
WebFlp-In™ Cell Lines are designed for rapid generation of stable cell lines that express a protein of interest from a Flp-In™ expression vector. These cells contain a single stably … can cats get sick from human fluWebFlp-In™-293 Human embryonic kidney 1 × 107 cells, frozen R750–07 Flp-In™-CV-1 African Green Monkey kidney 1 × 107 cells, frozen R752–07 Flp-In™-CHO Chinese Hamster … can cats get sick from moldWebSeveral Flp-In™ host cell lines which stably express the lacZ-Zeocin™ fusion gene and contain a single integrated FRT site are available (see page 7 for ordering information). To express your gene of interest in 293, CV-1, CHO, 3T3, BHK, or Jurkat cells, you may want to use one of the Flp-In™ host cell lines to establish can cats get sick from flea medicineWebFlp-In™ Cell Lines are designed for rapid generation of stable cell lines that express a protein of interest from a Flp-In™ expression vector. These cells contain ... The Flp-In … can cats get styesWebOct 25, 2024 · Flp-In-CHO:huOX40 cells as well as Flp-In-CHO:vector cells generated in-house (Pieris) were cultivated in Ham’s F12 medium (Gibco, #31765-027) with 10% FBS and 500 µg/ml hygromycin B. All mammalian cell lines were grown at 37°C and 5% CO 2. Goat anti-rabbit IgG-PE conjugate was purchased from Dianova/Jackson … can cats get spayed while pregnantWebFlp-In™ 细胞系设计用于快速生成稳定细胞系以从 Flp-In™ 表达载体表达关注的蛋白。这些细胞在转录活性基因组基因座处包含一个稳定整合的 FRT 位点。Flp-In™ 表达载体的靶向 … can cats get spayed while nursingWebJan 15, 2024 · We also established CHO cells expressing the WT protein using the Flp-In system as previously described (thereafter called Flp-In CHO-WT cells) [6]. The pcDNA5/FRT-PiT-2-flag in GFP vectors were obtained by inserting GFP into Loop7 of the SLC20A2 (WT, T115 M, and R467X) using in-fusion cloning system (Takara Bio Inc.; … can cats get sick from the cold